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KMID : 0043320110340122065
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Archives of Pharmacal Research
2011 Volume.34 No. 12 p.2065 ~ p.2071
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Multiple component quantitative analysis for the pattern recognition and quality evaluation of Kalopanacis Cortex using HPLC
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Men Chu-Van
Jang Yu-Seon
Lee Kwan-Jun
Lee Jae-Hyun
Quang Tran Hong
Long Nguyen Van
Luong Hoang Van
Kim Young-Ho
Kang Jong-Seong
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Abstract
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A quantitative and pattern recognition analyses were conducted for quality evaluation of Kalopanacis Cortex (KC) using HPLC. For quantitative analysis, four bioactive compounds, liriodendrin, pinoresinol O-β-D-glucopyranoside, acanthoside B and kalopanaxin B, were determined. The analysis method was optimized and validated using ODS column with mobile phase of methanol and aqueous phosphoric acid. The validation gave acceptable linearities (r > 0.9995), recoveries (98.4% to 101.9%) and precisions (RSD < 2.20). The limit of detection of compounds ranged from 0.4 to 0.9 μg/mL. Among the four compounds, liriodendrin was recommended as a marker compound for the quality control of KC. The pattern analysis was successfully carried out by analyzing thirty two samples from four species, and the authentic KC samples were completely discriminated from other inauthentic species by linear discriminant analysis. The results indicated that the method was suitable for the quantitative analysis of liriodendrin and the quality evaluation of KC.
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KEYWORD
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Kalopanacis cortex, Liriodendrin, HPLC, Linear discriminant analysis, Pattern recognition
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